Reverse complement what is

The orientation of primers means: the direction in which the elongation of the primer in DNA synthesis occurs. Since DNA synthesis is always from 5' to 3' , the 3' ends of a PCR primer set point towards each other, when they are annealed to their template strand, and the primers anneal on opposite strands of the PCR template. So the forward orientation is: pointing towards the end of a gene. Recognition sequences are written from 5' to 3', only one strand being given. If you're calculating the reverse complement by hand, be sure to double check!

It can be easy to miss a base pair or use the wrong complement, especially if you're reading a long sequence on paper. Helpful 0 Not Helpful 0. Submit a Tip All tip submissions are carefully reviewed before being published. Related wikiHows How to. How to. Solution by Gabriel. Co-authors: 3. Updated: May 6, Categories: Genetics.

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